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In a typical aqueous solution, the a large fraction of water will form ice crystals and the solutes will be concentrated in the remaining solution between the ice crystals.
Since the remaining solution (containing fenofibrate, mannitol, TBA, and water) is thermodynamically unstable, the solutes can start to crystallize as well.
Nucleic acids in the remaining solution were purified.
The remaining solution would be run through the laboratory's DNA sequencer.
This is dried and burned; the ash is leached with water; sodium sulfate and sodium chloride are removed by crystallization; and the remaining solution is concentrated by evaporation of water.
The remaining solution was centrifuged for 10 min and the precipitate was removed.
The remaining solution was kept at room temperature for 48 h to precipitate the fabricated microPCM.
A small aliquot was then set aside for pH determination and the remaining solution was filtered at Whatman 0.50 μm.
After removal of the EtOAc fraction, the remaining solution was then extracted with butanol for three times.
The ether was evaporated and the remaining solution was placed in a vacuum desiccator until being crystallized.
The root canal was irrigated with 1 mL of distilled water to wash out the remaining solution.
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CEO of Professional Science Editing for Scientists @ prosciediting.com