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The only hypothesis of population differentiation significantly supported by AMOVA was that between populations from Chapada Diamantina and the remaining localities (Table 4).
Based on the known geographical distribution of mitochondrial DNA we interpret mitochondrial DNA type for the remaining localities for which sequence data are not available.
Despite the strong genetic differentiation observed between populations from Chapada Diamantina and the remaining localities (Table 4), phylogeographic structure was not significant in E. secundum.
In contrast the population consisting of the remaining localities from clade 3 showed a more ragged distribution generally fitting a distribution expected under a growth/decline model.
Specifically, the estimated maturation reaction norms of cod from the two westernmost localities (1 and 2) are similar to each other and differ significantly from the remaining localities (Fig. 3B C).
The pattern for the remaining localities, of a majority of cases with darker shells in shaded habitat and a minority of cases with a reversed pattern, has been encountered before (Ożgo, 2005; Cook, 2008; Cameron and Cook, 2012a).
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For E. secundum, two additional models were considered, one corresponding to differentiation between populations from Chapada Diamantina and Planalto da Borborema, and the other considering populations from Chapada Diamantina separated from remaining localities.
Concerning nuclear markers, solely the RAG1 gene exhibited a differentiation and privatization of the five OTUs, even separating FP haplotypes in natator from remaining localities.
All individuals were glass eels caught by electrofishing (Iceland) and fyke nets (remaining localities).
Due to the larger population in the remaining two localities, two kindergartens were selected there.
The UNICEF Country Office will continue to advocate for adequate training of these Mediators and for the allocation of appropriate budget resources to provide this service to the remaining 27 localities densely populated by Roma.
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