This will influence the relative calculations.
For relative calculation the Delta Ct method was used [ABI PRISM® 7000 Sequence Detection System and Applied Biosystems 7500 Real-Time PCR System - User Bulletin, Applied Biosystems] once all assays met the amplification efficiency criteria of 100% ±10%[Application Note 127AP05-02].
Moreover, by combining the curved cylinder-sphere model with Li's model, the effect of pore structure, fluid distribution, and wettability can be incorporated into the relative permeability calculation model.
Results were expressed using the relative quantification calculation method in arbitrary units as described in [40].
For the relative risk calculation, the minimum proportion resistant among the Beijing strains was used.
Features for the relative risk calculation were analyzed as dichotomous variables (presence versus absence).
Note that the fairly high standard errors as indicated in the Whisker-Box plots in Figure 4 resulted from the inclusion of the PCR efficiency into the relative expression calculation of REST 2009.
The above description summarizes related work in the dependencies between aspects and the relative weight-age calculation issues of aspects.
The relative error between calculation and experimental value is within 2% in steady conditions and within 7% in unsteady conditions.
Moreover, PA0576 (rpoD) was used as a control gene for the relative mRNA level calculation due to the fact that rpoD exhibits stable expression level [ 18].
