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This strategy created plasmids that would produce FAX-1 DBD::heterologous LBD translational fusion proteins in which the region coding for the FAX-1 DBD was linked to an LBD-coding segment that began in the "hinge region" that separates DBD and LBD, and which replaced the FAX-1 LBD.
Targeted sequencing showed that all deletions were stable in the region coding for nsp2 after one swine passage.
Intrigued that their analyses spotlighted a gene near the region coding for the orexin receptor protein, which is known to be involved in the control of sleep, appetite and blood pressure, the team investigated further.
Based on these findings, we designed PCR primers to amplify the region coding for these differences to detect saa gene variants present in VTEC strains isolated in Argentina from cattle and meat.
Modern day serotype identification is based on virus isolation in cell culture and the nucleotide sequence of the region coding for VP1 protein.
To investigate this possibility, a number of constructs were made containing AUGi and the next AUG present in the region coding for C protein changed to other codons.
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In many genes, the regions coding for proteins are interrupted by intervening sequences of nucleotides called introns.
The sum of the lengths of these intervening sequences is sometimes longer than the sum of the regions coding for proteins.
When compared to the human ATM (hATM), the pATM showed a high level of homology in the coding region, particularly in the regions coding for functional domains, and had extensive alternative splicing of the 5'UTR, characteristic for the human ATM mRNA.
The NF90ctv gene and the regions coding for its deletions were amplified from pCI-neo/NF90 using specific primers (Table 1).
For the selection of other primers and probes, the regions coding for the genes investigated were retrieved from the GenBank and EMBL databases.
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