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Then, ratiometric data at each of the three comparisons using 'green' as the reference stage (i.e. pink/green; red/green; purple/green) were averaged prior to export for cluster analyses.
Two replicates of 70-mer oligo chips containing 19,200 features were hybridized to compare differential transcript abundance between each stage (2 through 7) and the reference (stage 1).
A total of 20 μg of the reference stage 1 (imbibed seed) (see Figure 1) RNA was used to be labeled by reverse transcription with Cy3-dUTP and compared to 20 μg of RNA from each of stages 2 7 labeled by reverse transcription with Cy5-dUTP following the protocol by Hedge et al., [ 60].
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"I thought if we were lucky and actually established something as a stage musical, the reference point for an eventual film of Wicked would become the musical, not the 1939 movie [The Wizard of Oz]," he reasoned.
The test consisted of two stages: first, the reference phase, warm-up (3-minute walk and stretching exercises) and second, the main test of ten laps, each 200 m long.
The "conceptual use" overlaps with "reference" stage of the research utilization ladder.
'Max' indicates where transcript was exclusively detected in gametocytes, 'Min' indicates where transcript was exclusively detected in the reference asexual stage and 'NA' indicates where transcript was not detected in either stage.
In this analysis the reference developmental stage was adult apterous aphids.
Using all the methods performed as the standard of reference, staging by F-FDG-PET was concordant with conventional staging procedures in 70 out of 88 patients (80%).
This paper presents the creation of a reference stage-gate process model for ideation activities in the early fuzzy front-end of new product development (NPD).
The greatest differences with respect to the reference condition (early stage) were observed at stage III (developing) (Figure 4; Dataset S4).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com