Sentence examples for the reference genes from inspiring English sources
The reproducibility and reliability of the results depend heavily on the reference genes used.
The transcript abundance of the reference genes was determined by the Ct value.
Summary of Illumina readsequencing reads mapped to the reference genes.
Our data suggest that the reference genes were expressed variably in the different samples.
The geNorm and NormFinder algorithms were used for validating the reference genes.
In order to allow for relative quantitation of gene expression, we used the reference genes 18S and GADPH.
Selection of the reference genes was based on the microarray results using an algorithm described in Popovici et al. (2009).
Expression values were calculated using the 2-ΔΔct method and normalised to the reference genes GPM1 and TDH3.
Oryza sativa OsUbi (Gene ID: 4332169) and M. oryzae Pot2 (Gene ID: 2680652) were used as the reference genes for, respectively, the host and the pathogen DNA.
As a result, use of these two pairs of co-regulated genes did not affect the final ranking of the reference genes by using geNorm software.
Also, the reference genes generally belong to a gene family and isogenes can be rather variably expressed in different tissues of different genotypes.
