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The intensity of Cy3/Cy5 ratio at each locus here will reflect the methylation status in the reference DNA relative to that in the DNA obtained from the site of the crime.
The reference DNA was prepared from the victim's blood.
The study sample and the reference DNA are labeled with similar but complementary BoBsTM probes.
Since the reference DNA has a T/S of 1.0, by definition, this slope is also the average TL of the reference DNA sample, 4,270 bp in our case.
The reference DNA is a pooled sample of DNAs from several normal Utah whites, aged 65 years or older.
The gene showing the greatest degree of methylation was FOXC1 with an average fold change of 60.9 versus the reference DNA.
Serial dilutions of genomic DNA from the Hela cancer cell line were used as the reference DNA to create the standard curve.
The quantities of telomeric product (T) and single copy gene (S) were determined relative to the reference DNA by the standard curve method.
The S signal is the number of nanograms of the reference DNA that matches the experimental sample for copy number of the single copy gene.
Genomic DNA from ULMs were used as the "test" DNA and matched myometria as the "reference" DNA during the hybridization reaction.
The reference DNA sample, corresponded to DNA obtained from the same cell line from which the nascent DNA preparation was originated.
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