Sentence examples for the recommended protocol from inspiring English sources
Brain: whole brains were incubated in CellEvent Caspase 3/7 Green (Thermo Fisher) according to the recommended protocol.
For the staining of Chop and T-bet, T cells were fixed with the Transcription Factor Fixation and Permeabilization Kit (eBioscience), using the recommended protocol from the vendor.
The acetic acid induced writhing is the recommended protocol for evaluating peripheral analgesic activity of medicinal plants.
Two micrograms of freshly isolated RNA were converted into first-strand cDNA as per the recommended protocol by using Superscript™ II reverse transcriptase (Invitrogen) and oligo-dT primer.
The total RNA was extracted from 3 × 107 leukocytes by the QIAGEN RNA Blood kit (QIAGEN, Mississauga, Canada) according to the recommended protocol.
We followed the recommended protocol described in the Affymetrix manual.
RNA extraction was performed with the RNeasy kit (QIAGEN®) according to the recommended protocol.
Luciferase activities were determined using Luciferase assay system (Promega, Madison, WI), according to the recommended protocol.
SureSelect in-solution hybridization was performed on the pooled samples using the recommended protocol for a single genomic DNA sample.
Total RNA was extracted using the PAXgene RNA Isolation kit (Qiagen, Valencia, CA, USA) following the recommended protocol.
Real-time quantification was performed in a 96-well plate using the IQ SYBR Green supermix and Chromo4 (Biorad, Hercules, CA, USA) with the recommended protocol.
