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This sequencing involves mapping of the reads on known annotated gene models but cannot be used to identify novel genes.
Genome coverage has been estimated by mapping the reads on the assembled genome using BWA v. 0.6.2-r126 [ 92] using default parameters and calculating coverage on a panel of 140 single copy genes.
Verification of the assembly was performed in three ways: 1) back-mapping of the reads on the assembled sequence used as reference, 2) comparison with sequences available in the GenBank, and 3) Sanger resequencing of several regions.
Despite making use of a large fraction of the original sequencing reads (65.41% of the intact sequence pairs -fragments- could be mapped to the contigs), the raw Trinity assembly was largely redundant, as the mapping of the reads on the assembled contigs revealed 75% of non-specific matches.
Of the reads, on average 60 % was derived from the targeted region.
The read density heatmaps show condensation distribution of the reads on a flowcell.
Similar(30)
The data read on the second switching cycle.
The rest, read on.
The half? Read on.
If this is the case, read on.
Meanwhile, the reading on current conditions was virtually unchanged.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com