The reader was set to transmit a query every second for 60 s.
The reader was set to hybrid mode, 160-kHz FM0, 30-dBm output power for 60 s.
The reader was set to transmit for 60 s, and the tag was moved away from the receiver until the receiver could not detect the EPC anymore.
The reader was set up as follows: mass range was set from 1,000 to 50,000 Da, optimized mass range was set from 1,000 to 15,000 Da, laser intensity was set at 265 and laser sensitivity was set at 7. The "All-in-one protein standard II" (Bio-Rad, USA) was used to obtain protein standard spectra for mass accuracy calibration.
The readers were set to the same mixer frequency.
The FLIM multiwell-plate reader was set up to excite the Gag CFP with a supercontinuum source and a 434/17 nm excitation filter, and the cells were imaged through a 482/35 nm emission filter.
The parameters of protein chip reader were set as follows: laser intensity was 150, detection sensitivity was 7, m/ z values range was from 0 to 20,000, optimization range was from 2000 to 20000, and maximum molecular weight was 50000 Da.
An automatic microtiter plate reader was set to zero according to the control wells.
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