These libraries are non-normalized, so we estimated the relative gene expression based on the quantity of reads.
When calling variants (especially from short reads), the quantity of reads and the accuracy of mapping the reads should be precise.
Note that some of the variation in the estimated abundances can be attributed to various factors including the quality of the sequenced genomes, the sensitivity of the error model (substitution, insertion or deletion errors), the quality of the generated reads and the disproportion in the quantity of the reads generated by MetaSim.
The quantity of sequencing reads enabled us to identify several novel EBV miRNAs and uncover the extensive presence of terminal and nucleotide variants of EBV miRNAs in NPC tissue.
We also alter expected coverage for the segments by estimating the quantity of mouse reads that will align (these values are fixed into WaveCNV, but can also be supplied as a BAM file if mouse was sequenced independently).
Moreover, the large quantity of reads allowed a draft genome for 15 species to be assembled.
We value quantity of reading over quality of reading.
Efficient base calling by using the latest Illumina data analysis pipeline software improved the quality and quantity of reads from the same raw image data.
We argue that full V(D J segmentation on these quantities of reads is unnecessary, and that a better strategy for clonality studies is to first cluster the reads derived from the same clone before the time-consuming V(D J segmentation.
New York Review Classics editorial director Edwin Frank, who joined Warner on the judging panel, admitted the judges had faced serious quantities of reading over the last year.
