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The assay for each matrix was linear within the quantification range with determination coefficient (r2) values above 0.995.
The serum assay showed good linearity within the quantification range 0.25 - 100 ng/mL for both stanozolol and 3′-hydroxystanozolol, with determination coefficient (r2) values higher than 0.9981.
Regression analysis indicated that the assay showed excellent linearity within the quantification range of 0.5 to 200 pg/mL water for stanozolol.
Correlation coefficients were greater than 0.99 for all nucleosides, such that the calibration curves showed good linearity within the quantification range.
The quantification range of this system was 4 orders of magnitude, from 103 to 106 copies per PCR, corresponding to the detection limit of 300 target copies per mL.
The quantification range of this assay was 6 orders of magnitude, from 8.9×101 to 8.9×106 copies per PCR, corresponding to the detection limit of 3.6×103 target copies mL−1.
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The latter generalization is known as "pervasion" (vyāpti; Tibetan khyab pa) of C by B a universally generalized material implication, for all x: if x is C then x is B, with the interesting feature that the quantification ranges over both actual and non-actual items.
OD260/OD280 of RNA used for the quantification ranged from 1.65 to 2.01.
Trueness of the methods was analyzed on two samples: 100% MON 810 leaf material (provided by CID-CSIC) on the upper range and 0.29% MON 810 maize flour (2C 2004/4 sample from USDA/GIPSA Proficiency Program) on the lower quantification range of the method.
The lower limit of quantification was 200 pg/mL and the linear quantification range up to 5,000 pg/mL in ascites as biological matrix.
A high background signal for blank control samples containing no analyte worsens the limits of detection and quantification for real samples and limits the linear quantification range of an assay.
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