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The quality and quantification of the RNA was checked and estimated by agarose gel electrophoresis and spectrophotometric analysis.
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The quality, integrity and quantification of total RNA were evaluated by spectrophotometric determination using a Bioanalyzer (Applied Biosystems, Foster City, CA, USA) and a NanoDrop ND-1000 (CelBio, Milan, Italy).
However, before comparing mRNA expression profiles across samples, correction for variables such as quality and quantification of the starting material and enzymatic efficiencies must be carried out [ 26- 28].
Total RNA was purified from each tissue, with assessment of RNA quality and quantification of purified RNA performed according to methods described previously [ 29].
An introduction to the engineering science of water quality characterization and quantification of treatment objectives.
Quality control and quantification of the libraries were performed using a DNA 1000 series II Bioanalyzer Chip (Agilent).
Each sample was run on the Agilent 2100 Bioanalyzer Nano LabChip for quality control and quantification of total RNA prior to reverse transcription into cDNA using the high capacity cDNA reverse transcription kit (Applied Biosystems, Vienna, Austria).
This chapter is an introduction to the engineering science of dirty water flow and quality characterization and quantification of treatment objectives for various end uses.
Quality control and quantification of DNA were performed before and after bisulphite conversion.
Quality assessment and quantification of cRNAs were performed with Agilent RNA kits on Bioanalyzer 2100.
Quality assessment and quantification of cRNAs were performed on Bioanalyzer 2100.
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