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The purpose of normalization is to reduce the effect of light condition for the definition evaluation.
The purpose of normalization is to correct changes in the nature of sediments, which may influence the contaminant distribution.
The purpose of normalization is to convert all attributes into real numbers in the range [0, 1].
Arguably, use of reference genes that display 2-fold changes in gene expression could serve the purpose of normalization, but only if the variance is technical in nature requiring additional measures (e.g., evaluation over time, etc) to establish suitability when using a single reference gene.
For the purpose of normalization, visceral adipose validated housekeeping gene ACTB was used as the reference [ 24].
For the purpose of normalization, the membranes were stripped, and re-probed with antibodies against β-actin (1 10,000; Sigma, St Louis, MO, USA).
Similar(43)
One of the purposes of normalization is to align the distribution of read counts across samples [ 6, 21, 22]; however, not all of the normalization methods successfully met this goal.
Negative and positive controls (vehicle, 1 µM NPY) were included on every plate for the purposes of normalization; for mutational studies, these controls were provided by the dual cell line expressing the relevant native Y receptor.
The correlation matrices not only provide a means to visualize sample heterogeneity, but also allowed us to define a most parsimonious set of blocks for each region for the purpose of data normalization.
The direct effect of poor normalization is evident when sample loading above 10 μg of a total protein lysate per lane is required because traditional loading HKPs such as GAPDH, actin, and tubulin are grossly overloaded and therefore not serving the purpose of data normalization [ 8, 9].
For the purpose of intensity normalization, each bin class (for each subject/position/nerve/side/slice) was divided by the bin class at which the histogram peak occurred in the age- and sex-matched control group (within the same respective slice/nerve/side).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com