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The purity was evaluated by measuring UV absorbance.
Total amount of RNA was quantified by optical density (OD) measurements using a ND-1000 Nanodrop Spectrophotometer (Thermo Scientific) and the purity was evaluated by measuring the ratio of OD at 260 and 280 nm.
The purity was evaluated on SDS-PAGE gels and the concentration was determined using the tyrosine difference method (Edelhoch, 1967).
The purity was evaluated via sodium dodecyl sulfate (SDS)–PAGE, and the concentration was determined using a theoretical molar extinction coefficient.
The purity was evaluated using a NanoPhotometer spectrophotometer (IMPLEN, CA, U.S .. RNA concentration was measured using a Qubit RNA Assay Kit and Qubit 2.0 Fluorometer (Life Technologies, CA, U.S .. RNA integrity was assessed using an RNA Nano 6000 Assay Kit and the Bioanalyzer 2100 system (Agilent Technologies, CA, U.S).
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DNA concentration was measured by spectrophotometry using the Nanodrop® 2000 (ThermoFisher, DE, USA) device and the DNA purity was evaluated using the A260/A280 and A260/A230 ratios.
The RNA purity was evaluated according the MIQE guidelines [ 24] by measuring the ratio A260/A280 with appropriate purity values between 1.8 and 2.0.
RNA was quantified spectrophotometrically at 260 nm, and the RNA purity was evaluated by measuring the ratio A260/A280, considering RNAs with appropriate purity those showing values between 1.8 and 2.0.
The RNA purity was evaluated by measuring the ratio A260/A280, considering RNA with appropriate purity those showing values between 1.8 and 2.0; its integrity was evaluated by gel electrophoresis.
Results: The effect of reducing agent concentration on the radiochemical purity was evaluated and optimum reducing agent amount found 200 μg.
The peak purity was evaluated by high-resolution mass spectrometry and LC-MS/MS.
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