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Careful endotracheal suctioning was performed before the protocol was started.
The protocol was started.
OES was performed every 15 minutes after the protocol was started.
The protocol was started in the ICU when patients met the inclusion criteria.
Subsequently, the slices were washed twice with Krebs solution, and the protocol was started.
The protocol was started upon arrival of the patients in the intensive care unit (ICU).
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Even in rodent strains that do not lose weight with EOD feeding, life span is increased when the protocol is started at young age.
To analyse the voltage dependence of activation, currents were evoked by 10-ms pulses ranging from −70 to +40 mV in steps of 10 mV and this protocol was started at 10 min after the recording was established.
As in the DirecNet study, the exercise protocol was started at 3 00 p.m., since this is the time of the day when many youth with type 1 diabetes participate in sports and other physical activities.
The measurement protocol was started when the recruited patients satisfied the following conditions: recovery of cough reflex; VT ≥ 8 ml/kg and respiratory rate ≤ 20 breaths/min; arterial blood gas of pH 7.35 7.45; PaCO2 35 45 mmHg; and arterial oxygen tension ≥ 100 mmHg at an inspired fraction of oxygen ≤ 0.5.
After the baseline recording, the experimental protocol was started.
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