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Last year, the protocol was extended to 2020, after which the new pact will take over.
Later on the protocol was extended to accommodate a wider spectrum of substrates, namely, triethyl orthothioesters and dithioorthoesters [ 45]. 2-nitroimidazoles, such as [F]EF3, are used to detect hypoxia, based on the bioreductive metabolism of the nitroimidazole pharmacophore.
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The protocol is extended to synthesize various important 12 laurate compounds with excellent yield (90 98%) and noteworthy recyclability (upto studied 5 recycles).
Given the wide interest in the preparation of functional heterocycles containing a trifluoromethyl unit, this protocol was extended to the synthesis of 4- and 6-trifluoromethyl-containing pyridines.
Meanwhile, the Kyoto Protocol was extended.
The optimized protocol was extended to a broader range of 22 target analytes that are relevant chemical markers, i.e., 15 carboxylic acids and 7 sugars.
The thermocycling protocol was extended by heating samples from 75°C to 95°C in 0.5°C increments for 15 sec, during which time the fluorescence data were collected in order to create a melt curve.
To identify minimal parameter adaptations that are necessary to describe a phenotype transition, the parameter estimation protocol was extended by including a regularization term given by the sum of squared parameter changes.
In 2010, the DVT surveillance protocol was extended to assess from lower extremities to include also the proximal upper extremities (axillary, brachial, cephalic veins) and internal jugular veins.
Therefore, the parameter estimation protocol was extended to avoid needless change of parameters, hereby identifying minimal parameter adaptations that are necessary to describe a phenotype transition.
As the signal threshold cycle (CT) is often delayed in cDNA from FFPE material the standard Taqman PCR protocol was extended to 50 cycles.
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