Although the protocol outlined here was developed for our original shRNA-based approach, it can be applied more generally, including to CRISPR-based approaches.
The protocol outlined in this study has been used to successfully predict compositions in a variety of alloy systems and has been validated with examples from the literature in Mg-, Al-, Ti-, Fe-, Ni-, Cu-, and Zr-based systems.
The default box size is calculated following the protocol outlined by the authors of Vina [34].
We used the protocol outlined by Luft and Roehrig (2007) to transcribe and code the interview recordings.
The review was conducted and reported according to the protocol outlined by PRISMA (Preferred Reporting Items for Systematic Review and Meta-Analyses) [4].
Trial management, population generation and phenotypic data collection of progenies comprising of 160 lines were performed using the protocol outlined under lowland conditions.
Electrophoresis experiments were performed following the protocol outlined in an earlier study.[25] In brief, crushed pyrite (Huanzala, Peru) was acid-washed and H2O-washed (both free of dissolved O2) under an N2 atmosphere.
Julie Werner-Simon, a former federal prosecutor who retired from DOJ in August 2015 after 29 years of service, argues that Comey's decision to make public an incremental development in the investigation with little clear significance is a breach of the protocol outlined in the manual.
As the method was established, different steps were optimized to generate the protocol outlined above.
Tagged-cDNA hybridization followed the protocol outlined in the 900 miRNA RT Kit.
PCR amplifications were carried out following the protocol outlined in the previous section.
