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The protocol is repeated for the opposite hemisphere.
Our results demonstrate a high degree of correlation between independent replicates (=.9446, Figure 3) and a narrow dispersion (σ2 = 0.00023, CV = 1.60%), suggesting that the amplification is highly reproducible and that the amplification bias is introduced consistently when the protocol is repeated.
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After Period 1, goats were switched to their new hay harvest times, and the protocol was repeated.
The protocol was repeated twice, each time with a handrim type: the standard round metallic tube handrims, and a prototype of an ergonomic handrim.
To then produce orthodromic action potentials, the protocol was repeated but with stimulation instead applied at the electrode that had recorded the largest somatic voltage peak for the respective neuron (see Fig. 1c).
The protocol was repeated three times on the same day.
In the second block the protocol was repeated but the song playback was reversed to control for any side biases.
The protocol was repeated for 40 cycles and included an initial 5 min enzyme activation step at 94°C and a final 10 min extension step at 72°C.
Duplicate half-gram manure pellets (both fresh and dry) were extracted using the alternate protocol of the MoBio UltraClean soil DNA isolation kit (MoBio, Solano Beach, CA) and the wash steps designated 15 to 21 in the protocol were repeated to remove a "green tinge" from DNA extract.
The protocol was repeated for untypeable isolates.
The protocol was repeated every 16.7 s.
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