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For immunofluorescence we followed the protocol described previously (Alvarez et al. Cereb. Cortex, 2016, 26, 1046 1058).
In vivo ubiquitination assay by agroinfiltration was performed following the protocol described previously.48 Coinfiltration expression of SGIP1 and SGS3 was performed in N. benthamiana leaves.
ChIP was performed following the protocol described previously (Schnekenburger et al., 2007).
Differentiation of H9 hESC into hNSC was followed by the protocol described previously (Liu et al., 2012).
The protocol described previously (Song et al., 2016) was used to detect the peptide(s) bound to the PXL2LRR protein by mass spectrometry.
A standard curve was constructed with genomic DNA from Clostridium cellulolyticum (DSM 5812) for primer cel48, following the protocol described previously [26].
We followed the protocol described previously [20].
HMVEC were cultured according to the protocol described previously [33].
The expression and purification followed the protocol described previously [11].
The small RNAs were cloned according to the protocol described previously [44].
For Cap-affinity assay we followed the protocol described previously [11].
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