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The protocol began with a baseline 8-h sleep episode at habitual bedtime, followed by a 16-h wake period.
The protocol began with a 10 min rest period to allow the participant to adjust to the research setting.
The protocol began at a pre-determined, comfortable walking speed for 3 minutes; the grade was increased by 2% each minute thereafter until volitional fatigue.
Power analysis was not performed for detecting changes in disease progression, as the percentage of strength decline was not known at the time the protocol began.
To complement serum creatinine, we obtained cystatin C levels with 33 of the total 84 renal ultrasounds but added this measurement after the protocol began.
The protocol began with an introduction and description of purpose based on existing studies using photovoice or photo-elicitation methodology, with an emphasis on health, women, and mothers.
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The protocol begins with 2 × 10 NK cells purified using the CliniMACS system, selecting CD3−CD56+ PBMCs.
Follow the same procedure as above for the remainder of the protocol, beginning with the Triton X-100 step during prehybridization.
The same happens when we talk about the use of cryptography of public key (almost all the protocols begin with the assumption that the participants know the public keys of the nodes with those that want to establish the communication).
The pharmacological protocol began at the completion of surgery with the mouse remaining under continuous anesthesia.
After 10 min warm-up at 70% of age-predicted maximal heart rate, the work protocol began at 9 km·h-1 and the speed was increased by 1 km·h-1 every minute so that exhaustion was reached in 8-12 min. Maximal oxygen uptake was considered to be the highest oxygen volume recorded during the last minute of exercise.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com