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The projected shoot area of rice cv.
The projected shoot area was calculated based on two side view images (at 90° from each other) and one top view image (Figure 1).
After 20 d of salt treatment, however, the projected shoot area of salt stressed rice was clearly reduced compared with that of the non-stressed plants, even at 50 mM NaCl.
There was a strong positive correlation between the projected shoot area obtained by image analysis and shoot fresh weight in the two rice cultivars, Fatmawati (R2 = 0.97) and IR64 (R2 = 0.98) (Figure 2) and there was no indication of any deviation from a linear relationship even at the highest biomasses measured in this experiment.
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To determine whether shoot biomass of rice plants correlated with the measurements of projected shoot area, and is therefore a quantifiable parameter that can be used to measure plant biomass, RGB images were obtained of plants that had been exposed to various salt stress levels for 20 d (Figure 1) before the fresh weight of each plant was determined by destructive harvest.
It is possible that the correlation between biomass and projected shoot area will still be strong for older plants, however, if running experiments on older plants then this should be confirmed, in case there are issues of areas of the plant being hidden from the camera by other parts of the plant.
Figure 3 Projected shoot area of rice cv.
Bars = 10 cm. Figure 2 Relationship between projected shoot areas and shoot fresh weight of rice.
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