The processing patterns of hairpin structured transcripts derived from relevant vectors could be different.
Furthermore, we assessed the processing patterns of these convertases in primary colon cancers.
This might be explained by the observation that the processing patterns for the tRNA class is not as coherent as for microRNAs.
In a study on the processing patterns of miRNA, Lee et al. profiled the expression of 202 mature miRNAs using qRT-PCR from 22 different human tissues [ 30].
Using RT PCR, immunoblot and immunohistochemistry we assessed the presence and the processing patterns of the convertases PC1 and PC2 as well as the PC2 specific chaperone 7B2 in human liver metastases originating from colorectal cancer and compared them to unaffected and normal liver.
The origin of the conserved processing pattern of mir-2 homologs is unclear.
To investigate the unusual processing pattern of L-Snord115, we subcloned the corresponding construct into the pcDNA 3.1 eukaryotic expression vector (fig. 2 A, sequence 1) and performed transient transfection experiments in HeLa cells.
The causing mechanisms of changes in the mRNA processing pattern involve both alteration of primary transcript regulatory sequences (cis-acting elements) and modifications in the activity of splicing factors (trans-acting elements).
The two fully processed transcripts are homologous to Caenorhabditis elegans Sod3 mRNA showing the same processing pattern.
Indeed, we found evidence for the P28 processing pattern both in rat and platypus (see Figure 4A, B), suggesting that conserved enzymatic processes generate the short by-products in species ranging from platypus to mouse.
