In brief, the labelling procedure consisted of assembling a reaction comprising 1000 nmol vancomycin HCl (MP Biomedicals), 250 nmol TMR, 300 nmol 1-ethyl-3- 3-dimethylaminopropyl -carbodiimide and 300 nmol 1-ethyl-3- 3-dimethylaminopropyl -carbodiimide 1-ethyl-3- 3-dimethylaminopropyl -carbodiimidetion 1-ethyl-3- 3-dimethylaminopropyl -carbodiimide.
Similar to the procedure of NCBI nr annotation, the assembled isotigs were searched against the TAIR9 protein database using the BLASTX software with default parameters.
The fabrication procedure of an assembled optical stretcher (AOS) is reported in detail elsewhere [ 6].
However, in many cases, it is crucial to restrain the warping deformations and maintain the coordinated displacements during the procedures of structural design, manufacturing, assembling and service.
a The snapshots of assembling two objects.
An example is displayed in Fig. 2D. Figure 2 (A B) SEM images that illustrate the procedure used to assemble two different types of spherical colloids (2.8-μm PS beads, 1.6-μm silica balls) into dimeric units.
The procedure involves the assembling of rat gene data, human gene data, rat-human gene orthology data and mouse-rat orthology data.
Heterojunction solar cells were assembled by covering the films synthesized at 400°C, 600°C, 800°C, and 1,000°C onto an n-type Si wafer with a square window of 3 mm × 3 mm surrounded by insulating silicon dioxide, and the detailed procedure of the cell assembling can be found in our previous report [23].
The degradation procedure works as follows: When it is necessary to degrade ongoing data calls, a cyclic process exists in which ongoing data calls with the largest amount of assembled channels are degraded first and calls with the least number of assembled channels are degraded last.
Through a sandwich-type DNA hybridization procedure, the target Salmonella DNA was captured and the indicator probe was assembled onto solid matrix so that the Salmonella DNA can be measured by the fluorescent signals of assembled indicator probes.
