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The mosquitoes were starved from sugar for 4-5 hours before conducting the probing time measurements as described in Lombardo et al., 2009.
Three independent sets of dsRNA injections for probing time were performed for each of the six genes and were compared to the probing time of GFP dsRNA injected control mosquitoes.
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During the bouncing of the probe, time varying signals are generated from optical mouse that are recorded in data files on PC.
If the mosquito terminates the probing unsuccessfully and tries again elsewhere, the second probing time is progressively added to the first one until blood is ingested.
Silencing of an apyrase gene in the salivary gland of A. gambiae resulted in an increase in the mosquito probing time as well as increase in vitro platelet aggregation [ 9].
To establish the detection method, we can optimize the step of the method as the amount of the IMB probe, time of IMS, the amount of QD probe, the label time, etc. [15, 25].
Thus, a simple self-serving pollinator behavior the adjustment of probing time in response to nectar volume may select against reducing nectar and protect many plant-pollinator mutualisms against a drift toward parasitism.
At present, several research efforts have addressed reducing the initial probe time [15, 21 26].
In this way, the tune-in time can be reduced to the initial index probe time plus the data-retrieval time.
For the probe trial, time (s) in quadrants, distance (cm) traveled in quadrants, time in annuli (s), distance (cm) traveled in annuli, time (s) at platform positions, and distance (cm) traveled at platform positions [ 71, 72] were analyzed.
During the bouncing of the probe, a time varying signal is generated from optical mouse that is recorded in a data file on PC.
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