Sentence examples for the primary template from inspiring English sources

Structural sizes in 3D ZnO structures obtained through templating processes depend highly on the sizes of the primary template.

To verify that plus strands were indeed the primary template for the PCR reactions, we incubated core DNA with sodium hydroxide to remove the RNA primer attached to the 5' end of plus strand DNA, thus removing 12 nts of the annealing site for primer 2477 during the PCR reaction (Table 1, experiment 5, Figure 3).

In mammalian cells, the sister chromatid is the primary template for HR compared to the homologous chromosome [ 20].

For second strand synthesis, PCR was carried out on a small aliquot (1/10th volume) of the primary template by using Advantage 2 Polymerase Mix (Clontech).

For the dopamine transporter example, we chose an amine transporter from Aquifex aeolicus VF5, identified by HHBlits with a sequence identity of ∼24%, as the primary template.

For second-strand synthesis, PCR was carried out using a small aliquot (1/10th volume) of the primary template and Advantage 2 Polymerase Mix (Clontech).

The T. cruzi maxicircle coding regions provide the primary templates for the RNA editing process.

However, these results were based on qualitative RT PCR, and differences in primer sensitivity and primary template concentrations could affect the levels of amplified product.

The second round was performed in a final volume of 25  μl with 2.5  μl of the primary PCR template as described above.

The size of the primary gene template encoded in the maxicircle is radically minimized, with up to half of a gene's coding information stored in gRNAs whose function is largely unaffected by transition mutations.

For degenerate primers, genomic DNA from the crown rust resistant Vedette6 genotype [ 14] was used as an primary template, and re-designed primer pairs were used with the F1 NA6 × AU6) parents.