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The flow cytometric intracellular cytokine staining assay is one of the primary assays for enumerating vaccine-induced T cells in vaccine clinical trials.
All the target compounds were evaluated in vitro for their antifungal activity against six phytopathogenic fungi, some compounds exhibited potential activity in the primary assays.
Some of the analogues displayed good activity in the primary assays, and the seven compounds 10b, 10c, 11e, 13e, 21, 22c and 22e (shown in Figure 1) were identified as the most promising candidates for further study.
Reverse transcription (RT) qualitative or quantitative PCR are the primary assays for waterborne hNoV monitoring.
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Again the length of incubation time may be selected simply to parallel that of the primary assay.
We identified two hits with validated structures and confirmed activity in the primary assay (IC50 values = 322 nM and 7.7 μM) and two orthogonal MEKK2 biochemical assays.
The primary assay analyzed RET exons 10, 11, 13, 14, and 16 with a total of seven reactions using eight unlabeled probes.
The primary assay genotyped wild-type exons, a common exon 13 polymorphism, and an exon 16 mutation, whereas other RET sequence variation was detected.
Many of the Pfizer assays were selectivity assays, aimed at removing "actives" from the primary assay where the activity was in fact due to cytotoxicity or another non-specific event.
When the primary assay did not produce >50% inhibition, the Ki value is treated as >10,000.
When the primary assay hit, but the secondary assay was not performed, the Ki value is also treated as >10,000.
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