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The protocol used to obtain polyphenolic rich extracts from bean samples was based on the previously method [ 32].
In this study, we modified the previously method of the combination of the suppressive subtractive hybridization and microarray techniques to differentially profile the low-expression transcriptomes of human hepatocellular carcinoma by directly labeling both of the reciprocal subtracted amplicons as target for cDNA microarray assays.
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And the population estimate from the study, announced late last year, was 765, a figure 2.5 times the estimate based on sightings of females and cubs, the previously used method.
The results (blue circles in Fig. 7(c)) agree with that of the previously described method.
The melting point was determined by the previously described method Thermo-FAD (Forneris 2009).
The tyrosinase activity was determined by the previously described method [10, 12, 31].
Extraction of the selected plants was prepared according to the previously reported method [21].
Then the separation of lymphocytes were carried out following the previously described method.
The conditions used for HPTLC were similar to those in the previously described method [12].
Immunofluorescence labeling of cells was performed according to the previously described method (Kobori et al. [1992]).
Total volatile basic nitrogen (TVB-N) content was determined according to the previously described method [12].
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