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Incorporation of the next base occurred after removal of the blocked 3' terminus and fluorescent tag of the previously incorporated nucleotide.
Alternatively, a more-ancestral co-transfer could be followed by successive within-region LGT events which over time superimpose DNA onto the previously incorporated region.
Further branching of the lesion bypass pathway occurs during nucleotide incorporations following the initial lesion loop-out in which the damage base can remain looped-out, leading to a frameshift mutation, or the primer can realign with the template, allowing the previously incorporated base to be positioned opposite the lesion.
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The court has previously incorporated this perspective in its opinions on the death penalty and life without parole for juvenile offenders.
We have previously incorporated the rtTA2S-S2 gencodingding a second-generation rtTA variant [ 6] and the tetO elements into the HIV-1 genome to control virus replication [ 21, 22].
In 2002, requiring an outcross stallion not closely related to her own horses, she purchased the Brazilian-foaled Jullyen El Jamaal, who has bloodlines not previously incorporated into the Varian program, as well as a line tracing back to Bay el Bey via Bey Shah.
The sequences from all EST libraries used for this study have been previously incorporated into the TIGR BtGI.
One such mutation, the 4995 mutation in DEN-4 NS3 at amino acid 158 (Ser→Leu), was previously incorporated into the DEN-4 vaccine candidate, rDEN4Δ30, and found to further attenuate the virus for SCID-HuH-7 mice and rhesus monkeys [ 17].
Single pass 5' and 3' reads for 169 full-CDS bFLICs were previously incorporated into the TIGR BtGI.
Based on the relative magnitudes of the regression coefficients, some newly incorporated features, such as 3′-UTR length, ORF length, and SA, contributed similarly to features previously incorporated in the context+ model, such as SPS, TA, and local AU.
For his "Hobbit" movies Mr. Jackson has previously incorporated Tolkien characters who didn't appear in the novel, like the elf Galadriel (who will be played by Cate Blanchett, reprising the role from the "Lord of the Rings" movies).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com