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Spoligotyping was performed by using a commercial kit (Isogen Bioscience BV, Maarsen, the Netherlands) according to the previously described method (29 ).
This study was designed to compare the reliability of a new computer based CT measurement to the previously described method to determine the tibial tubercle trochlear groove distance.
The tyrosinase activity was determined by the previously described method [10, 12, 31].
Then the separation of lymphocytes were carried out following the previously described method.
The results (blue circles in Fig. 7(c)) agree with that of the previously described method.
The melting point was determined by the previously described method Thermo-FAD (Forneris 2009).
Immunofluorescence labeling of cells was performed according to the previously described method (Kobori et al. [1992]).
Total volatile basic nitrogen (TVB-N) content was determined according to the previously described method [12].
In-gel digestion was conducted in accordance with the previously described method (Kim et al. 2006).
Immunofluorescence was performed on 20-μm coronal sections according to the previously described method [18].
The conditions used for HPTLC were similar to those in the previously described method [12].
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