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The present study complies with the World Health Organization and international guidelines on global surveillance [ 20].
Ethical standards: The present study complies with the current laws of the Netherlands.
The present study complies with the principles of the Declaration of Helsinki and the Regulating Norms and Directives for Research Involving Human Subjects formulated by the Brazilian National Health Council, Ministry of Health, established in October 1996.
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It is noteworthy that the data and statistical analysis used in the present study comply with the recommendations on experimental design and analysis in pharmacology, including that the data subjected to statistical analysis should have a minimum of n = 5 independent samples/individuals per group [19].
The procedure of the present study complied with the "Ethical Principles of Psychologists and Code of Conduct" (American Psychological Association, 2002).
The present study complied with the guidelines of the 2008 revision of the Helsinki Declaration.
The A. mellifera experiments performed in the present study comply with the current laws of Brazil.
All procedures used in the present study complied with the "Principles of laboratory animal care" from NIH. Primers for microsatellite markers were purchased from Eurogentec (Seraing, Belgium), or from Research Genetics (Huntsville, USA).
The present study complied with all applicable requirements of the United States and/or international regulations and was approved by the institutional review board of Brigham and Women's Hospital.
The present study complied with international standards for animal wellbeing and was approved by the animal experimentation ethics committee (Comitê de Ética em Experimentação Animal - CETEA) of the Federal University of Minas Gerais (Universidade Federal de Minas Gerais – UFMG) (protocol no. 157/2009).
As far as SAC, urea, cortisol and ir-inhibin are concerned, average values from the present study comply with those already documented for the bovine species (Matsuzaki et al. 2001; Carletti et al. 2007; Mooney et al. 2008; Mosher et al. 2013).
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