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Our results indicate that a systems approach coupled with a feedback loop could help overcome some of the present methodological limitations.
Using the present methodological approach, based on BuChE immunoprecipitation, we have demonstrated that plasma AChE activity levels are increased in AD patients.
Until this process becomes viable, the present methodological limitations have to be considered carefully in risk assessment.
In summary, the present methodological concept can be seen as complementary to more conventional methods to study the conservation of introns.
The present methodological study was embedded within a larger study conducted in March 2009 by Holubar and colleagues assessing the influence of referral bias on patient reported outcomes among patients treated for ulcerative colitis [ 8].
The aim of the present methodological study is to suggest a new way of evaluating physical activity of older adults by proposing different components underlying self-reported physical activity, namely intensity, muscle strength, turning actions and mechanical strain.
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On the whole, the presented methodological chain also appears of great interest for other scientific communities such as atmospheric science, oceanography, astrophysics and engineering.
Our results corroborate the presented methodological pipeline, the fundamental relevance of secondary structure consideration, as well as the elevated power and suitability of ITS2 in phylogenetics.
Most studies of gender differences in the ASD literature present methodological limitations regarding the treatment of dyadic data.
However, integrating these databases with the study data may present methodological challenges.
However, the size of practice populations and the small numbers of deaths present methodological difficulties.
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