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The preparations were checked according to their activity and stability.
The cell content and the purity of the preparations were checked by visual inspection using light microscopy.
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The purity of the preparations was checked by absorption spectra and pigment analysis via HPLC [ 22, 23].
The purity of the preparations was checked by SDS-PAGE analysis using a ready-made gel with 12% Tris-HCl (Bio-Rad, Perth, UK) and Coomassie staining, according to the manufacturer's instructions.
The quality of the preparation was checked by SDS-PAGE.
The polysaccharides were dissolved in 10% NaCl and the concentration of the preparation was checked using the phenol-H2SO4 method [ 84].
Purity of the preparation was checked by analytical HPTLC and negative ion matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) (Nimptsch et al. 2010).
The purity and integrity of the RNA preparations were checked spectroscopically and by agarose gel electrophoresis before carrying out the analytical procedures.
The DNA contamination in RNA preparations were checked in PCR assay with human glyceraldehyde-3-phosphate dehydrogenase (GAPDH 1 and GAPDH 2) primers and RNA as template.
dsRNA preparations were checked for integrity on an agarose gels stained with ethidium bromide.
Final preparations were checked by silver staining of SDS polyacrylamide gels and immunoblot with anti-SVOP or anti-FLAG antibodies.
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