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When in vivo data in these two cell lines were compared, there was a clear contrast in the preferential sequence for combination therapy.
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We suspect this is due to the preferential sequencing of primer dimers or to unperceived secondary structure in ITS2 sequences.
The reverse transcriptase may also have preferential sequence motifs as the RNA-DNA heteroduplex in the active center of HIV-1 reverse transcriptase [ 14] is of the A form; however, reverse transcriptase bias was not addressed in the currently described experiments.
In these few cases, insertion was not linked to the presence of a preferential sequence.
Utilization of preferential sequence around the TATA box, and coexistence with the Y Patch and REG are expected to elevate accuracy of prediction.
Our previous in vitro assays demonstrated that RIN can bind to sequences of C(C/T)(A/T 6(A/G G, and that the preferential binding sequence is 'CCA(A/T)(A/t)(A/T ATAG' [ 6].
As reported by Maitra [ 57], the rationale for preferential sequencing of the non-coding region of mtDNA has been the assumption that this portion of the mitochondrial genome is particularly susceptible to the rigors of DNA damage encountered in metabolically active cancer cells.
At the occurrence of the first and second voltage peaks, phenol was degraded by 20%and90%0%, respectively, suggesting a preferential sequence in substrate consumption.
Regarding the steps involved in protamine-induced activation, our results show that there is a preferential sequence of events.
Analysis of these results suggests that there is a preferential sequence of events during protamine-induced RyR2 activation.
The hnRNP proteins thus seem to be present throughout the length of pre-mRNPs and on individual transcripts they are likely to be present in specific combinations (reviewed in Singh and Valcárcel 2005), presumably determined by preferential sequence binding properties, post-translational modifications and cellular localisation.
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