To the tube containing residual biomass the solution with the appropriate enzyme loading (see Table 2) was added to equally replace the removed supernatant fraction, and the same predetermined quantity of biomass was included so that the hydrolysis could be repeated.
In this way, to operate near isothermal conditions and to minimize catalyst deactivation effects, a known quantity of methanol (10 ml) was dissolved into the isooctane as the solvent (250 ml) and the resulting mixture was charged into the reactor along with a predetermined quantity of catalyst (3 g).
Alginate beads were prepared that were loaded with 31% (w/w) Candida parapsilosis CCTCC M203011 cells {based on cell wet mass (cwm)}and 0.15 g of these cell-loaded alginate beads were added per mL of the aqueous phase, together with a predetermined quantity of co-substrate (0-258 mM based on the total volume of a biphasic system).
A predetermined quantity of MnO2 was added, and the stirring was continued for an additional 30 min.
The reactants of predetermined quantities are fed to the reactor and the reaction is conducted to produce the desired product of biodiesel.
The system has also allowed for 'bundling' of fees, with fees becoming flat when a predetermined quantity of services is exceeded.
About 2.0 g of wheat bran was taken in an Erlenmeyer flask and mixed with a predetermined quantity of Tris buffer (0.1 M, pH 8.0), and a calculated amount of beef extract and NaH2PO4 was also added to the flask.
By adding predetermined quantities of the radioactive chemical to the vials, investigators say, Simmons produced results that were in line with expectations.
Predetermined quantities of adsorbent were added to the bottles and initial solution pH was adjusted at desired value and mixing was achieved in the horizontal incubator shaker at 150 rpm and 37 °C temperature.
We modified filters of ten popular cigarette products with predetermined quantities (100 400 mg) of charcoal in a plug-space-plug configuration.
One way to provide a suitable incentive could be a "purchase fund" into which donors would pay money, and which would be legally obliged to buy predetermined quantities of any vaccine that met a set of criteria about effectiveness and appropriateness.
