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For the populations consisting of pure individuals, such as DH lines and recombinant inbreeding lines (RIL), probes with a high heterozygous proportion (>5%) and low minor allele frequencies (MAF < 0.01) may be filtered out, and materials/lines with high missing data (>20%) or low call rate (<0.7) may be discarded from further analysis [ 36, 40- 44].
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