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For insertions in the second part of the repeat, the majority was also in the "plus" orientation.
When the transposition reaction was performed at pH 7.0 or pH 8.0, more than 75% of total insertions in the first part of the repeat occurred with the transposon in the left to right, or the "plus" orientation relative to the 5' to 3' orientation of the GAA strand.
The CRM1-NES interaction is free from such spatial constraints as the entire exportin cargo complex enters the nuclear pore complex for transport to the cytoplasm, thereby allowing some NESs to bind CRM1 in the plus orientation and others in the minus orientation.
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Neurite outgrowth, length plus orientation tightly overlapped the pattern of the membrane surface.
In short: the intergenic region between araBAD and araC, with more than 100 bps of both flanking regions, was incorporated twice into the GFP reporter plasmid: once in the plus strand orientation (araC promoter) and once in the minus strand orientation (araBAD promoter).
Whether an NES binds CRM1 in the plus or minus orientation is determined by the location of its ΦXΦ strand motif.
In summary, we have found that NES peptides can bind the narrow CRM1-NES groove in two opposite orientations, which we now describe as the plus and minus orientations.
The existence of the potassium channel selectivity filter, plus the orientation of the glutamate binding domains to the transmembrane domains, defines this as a Group 1 channel.
When using the motif search program MEME [ 66] with all four promoters that SlMYC1 can activate, one 8 bp motif was identified in the plus or minus (for SlTPS9) orientation: CTAGG(T/A)(A/G G.
Detection analysis showed further advantages of the DSA approach with higher numbers of present calls than the Plus 2.0 array in both orientations.
The forward/reverse primers may fall on either the plus or minus strand, so long as the orientation relative to one another is appropriate.
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