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The plot was gated on live CD4+CD3+CD8− population.
The plot was gated on the live CD4+CD3+CD8− population.
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The plots are gated on hepatocytes by forward and side scatter and on live cells by excluding 7-AAD positive events.
The region representing the sporozoites on the scatter plots was gated and histograms of fluorescence intensity plotted for the gated population.
CD25brightCD4+ T cells and CD25-CD4+ T cells from synovial fluid were sorted according to the sorting gates shown in Fig. 4. The FACS plots are gated via CD3+ cells and the sorting gate for CD25brightCD4+ T cells includes all cells with a brighter CD25 expression than the CD25+CD8+ T cells.
The dot plots were gated on live CD3−CD19−NK1.1+ cells and the percentage of cells expressing CD49a and DX5 are shown.
The dot plots were gated on live CD3−CD19−NK1.1+ cells and display the percentage expressing CD49a and DX5 in each mouse strain in the liver (top panels) and the spleen (bottom panels).
The dot plots were gated on live CD3−CD19−NK1.1+ CD49a+DX5− liver trNK cells or CD3−CD19−NK1.1+ cNK9a−DX5+ cellsells in the liver for CD107a degranulation and TNFα production.
Representative dot plots were gated on live CD3−CD19−NK1.1+ cells and display the expression level of CD49a and DX5 in the liver (top panels) and the uterus (middle panels) and skin (bottom panels).
Dot plots are gated on CD8+ T cells; bar graphs depict the percentage of CD8+ T cells that are m45-tetramer+.
Flow cytometry outputs shown on dot plots were gated to exclude doublet signals.
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