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Despite the standardization of the phlebotomy procedure, blood analysis is occasionally requested after recommended hours with the excuse that the patient is still fasting.
In Bogotá, 30% of persons contacted did not keep their appointments, and although specific reasons for refusal were not always available, some of the drug users reported fear of receiving a positive blood test result, dread of the phlebotomy procedure, or apprehension of identification during the procedure.
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Clinical examination and sampling of biological specimens were performed using the guidelines of the National AIDS Control Program of Pakistan (http://nacp.gov.pk) and following the phlebotomy procedures as defined by the College of American Pathologists [ 14].
Details of the phlebotomy process in NHANES 1999 2006 are provided elsewhere (6).
The ability to extract adequate material for global molecular analyses from smaller clinical samples paves the way for less invasive sample collection techniques within the clinic, for example a 'finger pick' for blood versus a full phlebotomy procedure.
The nurse used standard phlebotomy procedures to obtain a fasting serum sample and a urine specimen.
Ten ml of peripheral blood was drawn from the subjects using standardized phlebotomy procedures in BD Vacutainer spray-coated K2EDTA Tubes (BD, Franklin Lakes, NJ).
Participants were required to fast overnight prior to donating approximately 20 ml of venous blood from the antecubital vein using standard phlebotomy procedures.
The samples were collected according to standard phlebotomy procedures from consented patients.
Blood samples for HIV viral load measurement were collected using routine phlebotomy procedures and analysed using the COBAS Ampliprep/Taqman platform (Roche Diagnostics Systems, Branchburg, NJ, USA).
Blood samples will be collected using standard venepuncture and phlebotomy procedures and samples will be transported to the local laboratory, where plasma/serum will be separated within 4 h of venepuncture.
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