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The phages were analysed using PHAST [ 76].
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Data were analysed using the Fiji Software34.
The amounts of the phages were normalized relative to wild type phage and then the phages were analysed in ELISA for quantification of scFv antibody fragment expressed together with FLAG peptide in recombinant phages, by using anti-lambda coated plates and an anti-FLAG AP-conjugated antibody.
Phage display clones are usually analysed using restriction digestion of PCR amplified VHH insert, followed by agarose gel-electrophoresis [ 4].
Two phage-displayed libraries were immunoscreened and the selected phage clones were analysed.
As controls, the absorbance of Fab fragment, phage particles (10 cfu/ml) displaying the Fab fragment and helper phage particles (10 cfu/ml) were analysed.
At the time phages were initially used for treatment of cholera, scientists had only just begun to study viruses and speculate about how phages work.
The reactivity of each of the typing phages with respect to the STEC O157 phage typing scheme was analysed.
The phage were stored at 4°C until further use.
The assembly of the Phi-191 sequence reads using as a reference the genome of the P13374 phage [GenBank: NC_018846.1] produced 151 contigs that were further analysed using bioinformatics resources.
Phages were directly incubated with a range of phage-degrading proteases, after which non-degraded phages were used for the next selection round.
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