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It also utilizes convex polygons to resolve the partial occlusion of a marker and properly enlarges the pattern area of a marker while adjusting its size without deteriorating the quality of marker detection.
Obviously, the larger the pattern area is, the more significant the use of a large writing field is, though at the cost of reduced resolution.
The corners show defects caused by fabrication imperfections since the pattern area is limited during holography and uneven distribution of resist during spin coating.
(4) Photoresist of 50 μm was spun on the Ni layer, and lithography was performed to develop the pattern area for Ni substrate.
(d) Photoresist of 10 μm was spun on the Ni layer, and lithography was performed to develop the pattern area for the Ni foil emitter.
Then, the sample with MUTMS patterns was incubated into 10 mmol L−1 HgCl2 solution for half an hour to form SH-Hg coupling, as shown in Fig. 3c, which will be used to immobilize cysteine-mutated CaM. 5 μg mL−1 CaM with buffer solution (25 mmol L−1 Tris HCl, 1 mmol L−1 CaCl2, pH 8.0) was deposited onto the pattern area for one hour in refrigerator at 4 °C (Fig. 3d) [32].
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In the former, the pattern, in small scale, is placed on a pattern drum, and feelers touching the pattern areas change the delivery rate of the pile feed roller.
There are many approaches for producing or modifying SAMs patterns and subsequent organization of the building blocks into the pattern areas.
After ion milling for 5 min, the outside and top of the pattern areas were removed, and only the Au sidewall structures (or Au boxes) remained on the substrates (Fig. 2c).
The PL intensity increases within the patterning area when the Au nanoplates with sizes of 150 and 100 nm are fabricated on top of the Si ND array.
Although these methods have many advantages including high patterning accuracy, their drawbacks include high cost and size limitations of the patterning area in practical applications.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com