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On the contrary, our data shows the overall modifications in these cells were not altered, even though the glutathione concentration was decreased 58%.
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The finding that minor alterations of the small Domain 2 (introduction of 5 residues in a domain of ∼50 amino acids) influence secretion was unexpected as we assume that the overall modification of the tertiary structure is minimal.
From Figure 4 it can be seen that the overall modification content correlates with the translational efficiency of the isolated tRNA ensemble, but the correlation is far from optimal.
In summary, we have reported the parallel quantification of 12 modified nucleosides in tRNA ensembles from various porcine and murine tissues and showed that the overall modification content varies substantially.
Indeed, the MS/MS analyses most likely reflect the overall modification status of PABP1 at different points within the cell cycle and within individual cells, as even synchronized cells contain multiple pI forms.
Overall, modifications of the GTV changed the treatment plan in 18 of 34 patients, and affected the percentage of total lung volume receiving >20 Gy in 25 of 34 patients.
Diamide shows an increase of overall modifications with time, whereas hydrogen peroxide shows a decrease of overall modifications with time.
Notably, besides the overall H3K4me3 modification density differences, the shape of H3K4me3 modification profiles differs among the three promoter types.
The ChIP data revealed subtle differences in the overall histone modification profile ("histone code").
Second, apparently, the overall histone modification profiles of latency cells were similar to that of uninfected CD4+ cells.
The overall histone modification patterns near HIV-1 integrated sites were not significantly different from those of control in this study.
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