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The overall directional distance for every miRNA is obtained by summing up these directional distances over all families.
The overall directional similarities implied that the genes that were sensitive to genomic disturbance tended to be highly responsive to external perturbation and vice versa.
To date, analyses of fitness landscapes have focused either on the overall directional curvature of the fitness landscape or on the distribution of pairwise interactions.
First, studies have used the overall directional curvature of mean fitness as a function of the number of random mutations introduced into the genome of some wild-type organism [ 2, 27- 31].
To date, these analyses have focused on the overall directional curvature of fitness as a function of the number of mutations, on the distribution of pairwise interactions, or on both.
Finally, as described for propagating slow-waves in the mammalian neocortex [ 9, 15], in general terms, the overall directional bias in hyperpallial plume propagation is parallel to the predominant orientation of myelinated axons in the zebra finch hyperpallium [ 36]; although, as noted above, individual plumes often propagate orthogonal to this axonal orientation.
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The analysis of median lifespan using the accelerated failure time model showed the same overall directional pattern as maximum lifespan – full controls had the longest lifespan, followed by both procedural control groups, and the four immune challenged groups suffered the shortest lifespan (Fig. 2, table 3).
In any case, the absence of overall directional curvature does not distinguish between two biological scenarios: (i) most mutations have independent effects such that there is very little epistasis; and (ii) epistasis is common but interactions between mutations are diverse in their directional effects, thereby obscuring overall average curvature [ 2].
A reminiscent mechanism was described in desert ants (Cataglyphis fortis) that return to nest directly but not necessarily in a straight path, presumably turning as frequently to the right as they do to the left, to reduce overall directional bias [ 29].
Consistent with the patterns for necrosis, there was no overall directional change after serial passage for the effect of inoculation on leaf survival (Fig. 2: barb34L: t10 = 0.26, P = 0.80; bifi4C: t10 = 1.41, P = 0.19).
Furthermore, we outline current mechanistic models about how dyneins walk along microtubules and maintain an overall directional bias toward the minus end.
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