Sentence examples for the original extracts from inspiring English sources

The original extracts were prepared at 200 mg ml 1 and further diluted with serum-free cell culture medium to obtain a series of diluted extracts (100, 50, 25, 12.5 and 6.25 mg ml 1).

For AEDA, the concentrated aromatic extract (200 μL) of CCA was 1 2 diluted stepwise using dichloromethane as the solvent to obtain serial dilutions (1 2, 1 4, 1 8 and up to 1 64) of the original extracts.

The original extracts, plus two dilutions (1 2 and 1 5) were printed in triplicate (printing replicates).

The products were analyzed by GC/MS using the same temperature programme as for the original extracts.

Then, culture medium was swapped for either fresh medium (negative control) or Zn-textile extracts in medium (original extract 100%, extraction ratio 1 g:50 mL) and serial dilutions of the original extract (75% at 0.75 g:50 mL, 50% at 0.5 g:50 mL, 25% at 0.25 g:50 mL, and 10% at 0.1 g:50 mL).

The original extracted DNA of samples was directly used without any DNA purification and amplification.

The original extract was obtained at 200 mg akermanite/ml LG-DMEM and further diluted with LG-DMEM.

The final extracts were denoted as 1/2, 1/4, 1/8, 1/16, and 1/32 extracts based on the concentrations of the original extract.

Feature selection was used to select a subset from the original extracted feature set in order to avoid performance degradation and high-computational complexity [46].

The original extract was spiked with 50 µl 0.1 % TFA in water, concentrated to a volume of 30 µl using a SpeedVac (Eppendorf) and subsequently applied to the ziptip sample preparation according to the manufacturer's protocol (Millipore, USA).

After extraction, a liquid liquid extraction and a clean-up step including the use of Florisil macrocolumns were required prior to injection of the extracts into the chromatograph in order to isolate the pesticide residues from the lipid fraction of the original extract.