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The rupture seems to have restarted near the origin between 80 s and 100 s, then decreased to −60 km in the next 30 s.
Additional arguments were worked out through the insertion of numerous textual insertions over the five revisions of the Origin between 1860 and 1872, including the addition of a new chapter dealing with "miscellaneous" objections to the sixth edition of the Origin.
Indeed, averaging spikes may enhance the signal-to-noise ratio but the differences in the origin between single spikes may get lost in the averaging process (Bast et al. 2004, 2006).
One of these (tentatively identified as the polar flagella cluster, PF) is located on chr1 between 993676 and 1046789 bp and the second cluster (tentatively identified as the lateral flagella cluster, Chr1.1-LF) is localized on chr1 near the origin between 13496 and 49254 bp.
The specific distribution and orientation of the FtsK Orienting Polar Sequences (KOPS) motif 5'-GGGNAGGG-3', which is involved in loading the FtsK DNA translocase and directing it to the replication terminus in α-proteobacteria [ 20], confirmed the predicted location of the origin between AZC_4717 and AZC_0001.
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The location of SPREAD origin was optimised by changing the ordinates of the SPREAD origin between −10 and 10 pixels and finding the position that gave the highest correlation efficiency between the data and the linear regression curve.
Interestingly, there is also a difference in gene expression between the sites that are equidistant from the origin, notably between left terminus (LT) and right terminus (RT) and right origin (RO) and left origin (LO).
Theoretically, the origin offset between these two orbits is associated with the geocenter motion.
We assess the impact of orbit modeling on the origin offsets between GRACE kinematic and reduced-dynamic orbits.
Approximately 57% of all transposase ORFs identified in strain CcI3 are found either near the origin, or between the 2 Mb to 2.5 Mb region.
The PFREUD_03690 gene for cas1/cas4 associated CRISPR proteins was found near the replication origin between 399 and 2000 bp but no CRISPR was associated with it.
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