On the basis that there is more sequence variability at the C-terminus of these proteins than at the N-terminus, where a completely conserved cysteine residue is always amino acid residue 2, the ButaIT/GNA fusion protein was designed with the scorpion toxin N-terminal to the lectin, with the expectation that this orientation would be more likely to result in the fusion protein retaining toxicity.
Scanning was performed to create maps of slices perpendicular to the substrate (XZ scans) since the structures in this orientation would be more biologically interesting, in terms of the arrangement of bacteria in relation to the substrate.
In a system where there is no variability in orientation, the frequency of occurrence at that orientation would be n(o)/n = 1, and therefore the orientation surprisal would be 0 bits.
If aligned correctly, the additive inverse of the rectangle's orientation would be the orientation of the device in the room.
Based on the field mapping studies, an optimal selection for the slice orientation would be an oblique pseudo-coronal plane with its frequency-encoding direction parallel to the field gradient measured from each subject.
If your company is already very relationship focused emphasizing collaboration, shared decision making, and interpersonal support a leader with the same orientation would be redundant.
While there have been certain progressive initiatives taken by the administration, the pro-corporate orientation would be evident to a ship in a fog.
Thus, if two spindle poles are in focus at the same z-plane, the estimated spindle orientation would be 0°.
It is possible that, if the PRI were moved farther away from the promoter region, orientation would be even less of a factor because there would be fewer constraints on DNA spatial arrangement such that the factors bound at the PRI could more easily be positioned with respect to the general transcription machinery.
On the remaining 25% of trials, a pair of gratings with the orthogonal (unexpected) orientation would be presented (Fig. 1C).
