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The optimized procedure was applied to study of metabolic stability of CVA.
The accuracy of the optimized procedure was evaluated by the analysis of certified reference materials.
A confirmatory experiment was conducted to evaluate the accuracy of the optimized procedure.
The optimized procedure easily determined codeine and paracetamol with separation in less than 3 min.
The optimized procedure involves heating the gelatin and gelatin glycerol solutions up to 90 °C.
The optimized procedure gave recoveries of spiked PP7 of 64±4.8% (mean±SEM).
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The optimized procedures were thoroughly validated and applied for determining the enzyme's kinetic parameters and the inhibition characteristics of two potent probe inhibitors.
Lastly, we evaluated the growth, surface markers and differentiation properties of DPSC obtained from intact teeth and undigested, whole dental tissue frozen and thawed using the optimized procedures.
The optimized procedures described here will greatly benefit the malaria genomic research and control initiatives that have faced serious challenges with the parasite's extreme AT-rich genome.
For this comparison, both free and total plasma sulfide (free + acid labile + bound sulfur) was quantified using the optimized procedures for the mBB assay from identical samples from the same mice.
The smoothed nature of the video traffic generated using the described optimized procedure also affects the transmission buffer load.
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