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The optimized method was validated and excellent results were obtained.
The optimized method was successfully applied to estimate CVA metabolic stability in RLMs.
The optimized method was found to be efficient, precise, accurate, specific, and economic.
Validation of the optimized method was performed according to ICH Q2 (R1) guidelines [29].
The optimized method was tested at a field site contaminated with chlorinated solvents.
The optimized method was applied for the determination of targets in surface water samples.
The optimized method was validated for selectivity, linearity, limits of detection and quantification, recovery and precision.
The optimized method was applied to grapes and to winemaking by-products.
The optimized method was used for the quantification of trace minerals in a certified seawater sample.
The optimized method was used to determine resveratrols among three different cultivars.
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According to the delay between two image captures (2 h in our case), it is interesting to observe that the correlation can be computed between two captures only if the optimized method is used.
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