Sentence examples for the optimized gene was from inspiring English sources

The optimized gene was custom synthesized by Genomics company, Taiwan.

The optimized gene was then synthesized by Entelechon and finally cloned into pIVEX2.3 (Roche, Germany).

Compared with the wild-type gene, the optimized gene is expressed at a significantly higher level in P. pastoris after methanol induction for 168 h in 5- and 50-L bioreactor with the maximum activity of 8100 and 11000 U/mL, which was 2.31- and 2.62-fold higher than that by wild-type gene.

The gene annotation enrichment analysis for the optimized genes was taken by DAVID [ 53], where the tools can provide functional interpretation of large gene lists derived from genomic studies.

We found that 31 of the optimized genes were targeted as drug response markers in DrugBank.

An analysis of expression profiling data revealed that many of the optimized genes were expressed differentially in the four cancers.

The optimized genes were also annotated to cancer pathways, and to the Notch signaling pathway and the cell cycle in KEGG.

A function enrichment analysis for GO and KEGG indicated that the optimized genes were mainly involved in the biological processes of regulating cell apoptosis and programmed cell death.

We also found that the optimized genes were mainly enriched in the GO terms involving regulation of gene expression, regulation of transcription and in the biological processes of cell apoptosis and programmed cell death (Additional file 9).

The optimized genes are also classified as four types according to the number of cancer types that the genes connected; 79 optimized genes for type I, 12 for type II 26 for type III and 37 for type IV (Additional file 4).

Thus, the accumulation level of the enzyme with the plant-expression optimized gene was about 5 fold higher than obtained with the human sequence.