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Therefore, the inhibition of the catalytic activity of the His332Glu mutant is because of the structural changes of the OEC itself during the assembly process.
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In moderate heat stress or stronger (≥40 °C), a pronounced K-step due to irreversible severe damage on the OEC occurred.
In contrast, due to the SFE at 35 MPa showed lower mass transfer rate than 20 MPa; then, the extraction of p-coumaric acid and ferulic acid derivative II from lees continues along the OEC. Figure 6 shows the OECs of the more polar polyphenols: gallic acid, protocatechuic acid, quercetin and quercetin derivative II, and its concentration in the extracts along the OECs.
This indicated that the OEC was damaged and the energetic connectivity between photosynthetic units was changed [ 48].
Yield was calculated along the OECs.
The OECs were determined in duplicate.
In general, SFE extracts showed the same polyphenols profile throughout the OECs.
Immunocytochemistry revealed that 96.8±0.5% of GFP-expressing OECs were labeled for the OECs marker S100 before grafting (Fig. 8A).
Moreover, Boyden chamber migration assay indicated that TNF-α was a chemoattractant for OECs migration, and TNF-α blocking with antibody greatly decreased the OECs migration activity.
As shown in Fig. 7A, the OECs migration-promoting effect of glial scar tissue was time-dependent.
Finding the in vivo counterpart for the OECs will resolve this argument, however such a discovery remains challenging due to the rarity of OECs in peripheral blood.
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